New enzymatic method for l-arginine determination based on use of human arginase i and urease

ScienceRise

Наталія Євгенівна Стасюк¹  Михайло Васильович Гончар²  Галина Зуфарівна Гайда³  Софія Ростиславівна Басс⁴  Хасмік Суренівна Єпрємян⁵ 

[1] Institute of Cell Biology NAS of Ukraine 14/16 Drahomanov str., Lviv, Ukraine, 79005;Institute of Cell Biology NAS of Ukraine, 14/16 Drahomanov str., Lviv, Ukraine, 79005;Institute of Cell Biology, NAS of Ukraine, 14/16 Drahomanov Str., Lviv, Ukraine, 79005;Ivan Franko National University of Lviv4 Gryshevskoho str., Lviv, Ukraine, 79005;Scientific and Production Centre “Armbiotechnology” NAS of Armenia, 14 Guriyan str., Yerevan, Armenia, 0056;

关键词: enzymatic assay;    arginase I;    urease;    o-Phthalaldehyde;    L-arginine;    urea;    ammonium;    spectrophotometry;   

DOI  :  10.15587/2313-8416.2015.45126

来源: DOAJ

【 摘 要 】

It is developed a sensitive and selective method for quantitative analysis of L-arginine with spectrophotometric method of detection of the reaction product (ammonium) using o-Phthalaldehyde. The method is based on the use of highly purified arginase I and commercial urease. The new method is highly sensitive for L-arginine determination, a wide range of linearity and insensitivity to interfering influence of wide range of amino acids

【 授权许可】

Unknown