The Antibacterial Effects of Quaternary Ammonium Salts in the Simulated Presence of Inhibitors in Root Canals: A Preliminary In-Vitro Study

Coatings

HockinH. K. Xu¹  MichaelD. Weir¹  Biao Ren²  Xian Peng²  Mingyun Li²  Yannan Huang²  Lei Cheng²  SanjayKumar Tiwari²  Xuedong Zhou²  Suping Wang³ 

[1] Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, MD 21201, USA;State Key Laboratory of Oral Diseases, West China school of Stomatology, Sichuan University, Chengdu 610041, China;Stomatology Center, The First Affiliated Clinical Medical College of Zhengzhou University, Zhengzhou 450052, Henan, China;

关键词: quaternary ammonium salt;    dimethylaminododecyl methacrylate;    dimethylaminohexadecyl methacrylate;    root canal inhibitors;    antibacterial effects;   

DOI  :  10.3390/coatings10020181

来源: DOAJ

【 摘 要 】

To investigate the antibacterial effects of two newly developed quaternary ammonium salts (QAMs)-dimethylaminododecyl methacrylate (DMADDM) and dimethylaminohexadecyl methacrylate (DMAHDM), in the presence of various root canal inhibitors. Streptococcus gordonii, Enterococcus faecalis, Lactobacillus acidophilus and Actinomyces naeslundii were used. Dentine, dentine matrix and dead bacteria were selected as inhibitors. The antimicrobial efficacy of monomers of DMADDM and DMAHDM was tested against suspensions formed by mixtures of four bacterial species in or without the presence of inhibitors. The inhibition results were compared with chlorhexidine (CHX) and sodium hypochlorite (NaOCl). One-way analyses of variance (ANOVA) followed by Tukey’s multiple comparison test was performed to determine significant differences. The antibacterial effects of DMADDM and DMAHDM were variably inhibited dentine, dentine matrix and dead bacteria. CHX and NaOCl showed substantivity and they inhibited bacteria present in suspension. The concentration of compound decreased in the dentine block due to constant release. Bacterial colonies on the dentine surface and dentine tubules were significantly inhibited by DMADDM and DMAHDM. The antibacterial effects of DMADDM and DMAHDM could be inactivated by dentine, dentine matrix and dead bacteria, while DMADDM and DMAHDM could inhibit bacteria colonization on the dentine surface and kill bacteria present in dentinal tubules. The antibacterial effects of DMADDM and DMAHDM as free monomers in the presence of inhibitors was evaluated for the first time. They could help inhibit the residual bacteria on the dentine surface and in dentinal tubules that may cause persisting infection. Therefore the novel QAMs showed great potentials as root canal medication.

【 授权许可】

Unknown