Development of Real-Time Transendothelial Electrical Resistance Monitoring for an in Vitro Blood-Brain Barrier System

Micromachines

Kai-Hong Tu¹  Han-Yi Hsu¹  Zong-Han Sie¹  Ya-Yu Chiang¹  Ling-Shan Yu²  Julie Tzu-Wen Wang³  Khuloud T. Al-Jamal³ 

[1] Department of Mechanical Engineering, National Chung Hsing University, Taichung 402701, Taiwan;Rapid Screening Research Center for Toxicology and Biomedicine, National Sun Yat-sen University, Kaohsiung 813016, Taiwan;School of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King’s College London, London SE5 9NU, UK;

关键词: transendothelial electrical resistance;    TEER;    in-situ monitoring;    3D cell culture;   

DOI  :  10.3390/mi12010037

来源: DOAJ

【 摘 要 】

Three-dimensional (3D) cell cultures and organs-on-a-chip have been developed to construct microenvironments that resemble the environment within the human body and to provide a platform that enables clear observation and accurate assessments of cell behavior. However, direct observation of transendothelial electrical resistance (TEER) has been challenging. To improve the efficiency in monitoring the cell development in organs-on-a-chip, in this study, we designed and integrated commercially available TEER measurement electrodes into an in vitro blood-brain barrier (BBB)-on-chip system to quantify TEER variation. Moreover, a flowing culture medium was added to the monolayered cells to simulate the promotion of continuous shear stress on cerebrovascular cells. Compared with static 3D cell culture, the proposed BBB-on-chip integrated with electrodes could measure TEER in a real-time manner over a long period. It also allowed cell growth angle measurement, providing instant reports of cell growth information online. Overall, the results demonstrated that the developed system can aid in the quantification of the continuous cell-pattern variations for future studies in drug testing.

【 授权许可】

Unknown