Activation of Local and Systemic Defence Responses by Flg22 Is Dependent on Daytime and Ethylene in Intact Tomato Plants

International Journal of Molecular Sciences

András Kukri¹  Irma Tari¹  Zalán Czékus¹  Attila Ördög¹  Péter Poór¹  Gabriella Szalai²  Kamirán Áron Hamow² 

[1] Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary;Department of Plant Physiology, Agricultural Institute, Centre for Agricultural Research of the Hungarian Academy of Sciences, 2462 Martonvásár, Hungary;

关键词: dark;    ethylene;    flagellin;    jasmonic acid;    night;    reactive oxygen species;   

DOI  :  10.3390/ijms22158354

来源: DOAJ

【 摘 要 】

The first line of plant defence responses against pathogens can be induced by the bacterial flg22 and can be dependent on various external and internal factors. Here, we firstly studied the effects of daytime and ethylene (ET) using Never ripe (Nr) mutants in the local and systemic defence responses of intact tomato plants after flg22 treatments. Flg22 was applied in the afternoon and at night and rapid reactions were detected. The production of hydrogen peroxide and nitric oxide was induced by flg22 locally, while superoxide was induced systemically, in wild type plants in the light period, but all remained lower at night and in Nr leaves. Flg22 elevated, locally, the ET, jasmonic acid (JA) and salicylic acid (SA) levels in the light period; these levels did not change significantly at night. Expression of Pathogenesis-related 1 (PR1), Ethylene response factor 1 (ERF1) and Defensin (DEF) showed also daytime- and ET-dependent changes. Enhanced ERF1 and DEF expression and stomatal closure were also observable in systemic leaves of wild type plants in the light. These data demonstrate that early biotic signalling in flg22-treated leaves and distal ones is an ET-dependent process and it is also determined by the time of day and inhibited in the early night phase.

【 授权许可】

Unknown