Gene Modules Co-regulated with Biosynthetic Gene Clusters for Allelopathy between Rice and Barnyardgrass

International Journal of Molecular Sciences

Chuyu Ye¹  Sanling Wu¹  Fangjie Liu¹  Hongyu Chen¹  Lingfeng Mao¹  Yingying Wang¹  Jie Qiu¹  Most.Humaira Sultana²  Dongya Wu²  Longjiang Fan²  Qian-Hao Zhu²  Fei Fu²  Weidi Wang²  Lei Jia²  Md. Alamin² 

[1] Institute of Bioinformatics, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;;Institute of Crop Science &

关键词: allelopathy;    rice;    barnyardgrass;    biosynthetic gene cluster;    WGCNA;   

DOI  :  10.3390/ijms20163846

来源: DOAJ

【 摘 要 】

Allelopathy is a central process in crop−weed interactions and is mediated by the release of allelochemicals that result in adverse growth effects on one or the other plant in the interaction. The genomic mechanism for the biosynthesis of many critical allelochemicals is unknown but may involve the clustering of non-homologous biosynthetic genes involved in their formation and regulatory gene modules involved in controlling the coordinated expression within these gene clusters. In this study, we used the transcriptomes from mono- or co-cultured rice and barnyardgrass to investigate the nature of the gene clusters and their regulatory gene modules involved in the allelopathic interactions of these two plants. In addition to the already known biosynthetic gene clusters in barnyardgrass we identified three potential new clusters including one for quercetin biosynthesis and potentially involved in allelopathic interaction with rice. Based on the construction of gene networks, we identified one gene regulatory module containing hub transcription factors, significantly positively co-regulated with both the momilactone A and phytocassane clusters in rice. In barnyardgrass, gene modules and hub genes co-expressed with the gene clusters responsible for 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) biosynthesis were also identified. In addition, we found three genes in barnyardgrass encoding indole-3-glycerolphosphate synthase that regulate the expression of the DIMBOA cluster. Our findings offer new insights into the regulatory mechanisms of biosynthetic gene clusters involved in allelopathic interactions between rice and barnyardgrass, and have potential implications in controlling weeds for crop protection.

【 授权许可】

Unknown