High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae)

Frontiers in Plant Science

Ziwei Mei³  Yujun Shi⁴  Jianbo He⁵  Jiangjie Lu⁶  Yijun Meng⁷  Huizhong Wang⁷  Chenjia Shen⁷  Pengli Liu⁷  Yuyang Liu⁷  Shangguo Feng⁷  Jing Xu⁸  Xiaotong Wang⁸ 

[1] Center of Rare Plant Medicine Research of Zhejiang Province, Wuyi, China;College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China;College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China;School of Foreign Languages, Zhejiang Gongshang University, Hangzhou, China;Soybean Research Institute, Nanjing Agricultural University, Nanjing, China;State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China;Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou, China;Zhejiang ShouXianGu Pharmaceutical Co. Ltd., Wuyi, China;

关键词: Dendrobium;    Genetic linkage map;    Polysaccharides;    Quantitative trait locus (QTL);    SNP;   

DOI  :  10.3389/fpls.2018.00398

来源: DOAJ

【 摘 要 】

Plants of the Dendrobium genus are orchids with not only ornamental value but also high medicinal value. To understand the genetic basis of variations in active ingredients of the stem total polysaccharide contents (STPCs) among different Dendrobium species, it is of paramount importance to understand the mechanism of STPC formation and identify genes affecting its process at the whole genome level. Here, we report the first high-density single-nucleotide polymorphism (SNP) integrated genetic map with a good genome coverage of Dendrobium. The specific-locus amplified fragment sequencing (SLAF-seq) technology led to identification of 7,013,400 SNPs from 1,503,626 high-quality SLAF markers from two parents (Dendrobium moniliforme ♀ × Dendrobium officinale ♂) and their interspecific F1 hybrid population. The final genetic map contained 8, 573 SLAF markers, covering 19 linkage groups (LGs). This genetic map spanned a length of 2,737.49 cM, where the average distance between markers is 0.32 cM. In total, 5 quantitative trait loci (QTL) related to STPC were identified, 3 of which have candidate genes within the confidence intervals of these stable QTLs based on the D. officinale genome sequence. This study will build a foundation up for the mapping of other medicinal-related traits and provide an important reference for the molecular breeding of these Chinese herb.

【 授权许可】

Unknown