Molecular Cytogenetic Analysis of the Introgression between Agropyron cristatum P Genome and Wheat Genome

International Journal of Molecular Sciences

Liqiang Song¹  Zhi Zhang²  Shenghui Zhou²  Weihua Liu²  Xinming Yang²  Xiuquan Li²  Lihui Li²  Jinpeng Zhang²  Haiming Han²  Yida Lin² 

[1] Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology of Sciences, Shijiazhuang 050022, China;National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;

关键词: common wheat;    Agropyron cristatum;    translocation line;    introgression;    stripe rust;   

DOI  :  10.3390/ijms222011208

来源: DOAJ

【 摘 要 】

Agropyron cristatum (2n = 4x = 28, PPPP) is an important wild relative of common wheat (Triticum aestivum L., 2n = 6x = 42). A previous report showed that the wheat-A. cristatum 6P translocation line WAT655 carrying A. cristatum 6PS (0.81–1.00) exhibited high resistance to prevalent physiological races of stripe rust (CYR32 and CYR33). In this study, three disease resistance-related transcripts, which were mapped to A. cristatum 6PS (0.81–1.00) through the analysis of specific molecular markers, were acquired from among A. cristatum full-length transcripts. The BC₅F₂ and BC₅F_2:3 genetic populations of the translocation line WAT655 were analyzed by using three disease resistance-related gene markers, A. cristatum P genome-specific markers, and fluorescence in situ hybridization (FISH). The results revealed that the introgression between A. cristatum P genome and wheat genome was observed in progenies of the genetic populations of the translocation line WAT655 and the physical positions of the three genes were considerably adjacent on A. cristatum 6PS (0.81–1.00) according to the FISH results. Additionally, kompetitive allele-specific PCR (KASP) markers of the three genes were developed to detect and acquire 24 breeding lines selected from the progenies of the distant hybridization of wheat and A. cristatum, which showed resistance to physiological races of stripe rust (CYR32 and CYR33) and other desirable agronomic traits according to the field investigation. In conclusion, this study not only provides new insights into the introgression between A. cristatum P genome and wheat genome but also provides the desirable germplasms for breeding practice.

【 授权许可】

Unknown