Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay

Parasite Epidemiology and Control

Mónica Ozorio¹  Ninfa Vera de Bilbao²  Martha Torales²  Luis Ferreira²  Beatriz Trinidad³  Nidia Martínez³  Florencia del Puerto⁴  Luciano Franco⁵ 

[1] Corresponding author at: Department of Tropical Medicine, Research Institute in Health Science (IICS-UNA), National University of Asunción, Campus UNA, San Lorenzo, Paraguay.;Department of Entomology, National Service of Malaria Elimination (SENEPA), Ministry of Public Health and Social Welfare, Asunción, Paraguay;National Program of Malaria Control, National Service of Malaria Elimination (SENEPA), Ministry of Public Health and Social Welfare, Asunción, Paraguay;National University of Asunción, Research Institute in Health Science (IICS-UNA), Department of Tropical Medicine, San Lorenzo, Paraguay;Technical Management Office, National Service of Malaria Elimination (SENEPA), Ministry of Public Health and Social Welfare, Asunción, Paraguay;

关键词: Malaria;    PCR;    Paraguay;   

DOI  :  

来源: DOAJ

【 摘 要 】

In Paraguay, no cases of Malaria have been recorded since 2011. Microscopy and the SnM-PCR technique were implemented to detect and characterize Plasmodium spp. both in mosquitoes and in humans residing in historically endemic areas of Paraguay, to evaluate the possibility of finding asymptomatic cases and/or Plasmodium parasites circulating in anophelines. Between 2013 and 2015, 361 human blood samples were collected on filter paper, and between 2016 and 2017, 938 female Anopheles mosquitoes were captured in 15 Paraguayan localities. All the diagnostic techniques showed negative results. We observed no asymptomatic case or Plasmodium circulating in vectors.

【 授权许可】

Unknown