Treatment With Hydrolyzed Diet Supplemented With Prebiotics and Glycosaminoglycans Alters Lipid Metabolism in Canine Inflammatory Bowel Disease

Frontiers in Veterinary Science

Sebastian Neuber¹  David Borts¹  Viet Dang¹  Udo Müller²  Barbara Glanemann²  Yeon-Jung Seo³  Albert Jergens³  Yoko M. Ambrosini³  Dana Borcherding³  M. Gordian Adam⁴  Jonathan P. Mochel⁵  Auriel A. Willette⁶  Karin Allenspach⁶  Todd Atherly⁷  Oliver A. Garden⁸  Sergi Segarra⁸ 

[1] Biocrates Life Sciences AG, Innsbruck, Austria;D Bioiberica S.A.U., Barcelona, Spain;Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, United States;Department of Clinical Studies and Advanced Medicine, University of Pennsylvania College of Veterinary Medicine, Philadelphia, PA, United States;Department of Food Science and Human Nutrition, College of Human Sciences, Iowa State University, Ames, IA, United States;Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, United States;Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United States;;R&

关键词: metabolomics (OMICS);    inflammatory bowel disease;    dogs;    serum;    partial least-squares-discriminant analysis;   

DOI  :  10.3389/fvets.2020.00451

来源: DOAJ

【 摘 要 】

Canine inflammatory bowel disease (IBD) is a chronic, immunologically mediated intestinal disorder, resulting from the complex interaction of genetic, environmental and immune factors. Hydrolyzed diets are used in dogs with food-responsive diarrhea (FRD) to reduce adverse responses to immunostimulatory proteins. Prebiotics (PRBs) and glycosaminoglycans (GAGs) have previously been demonstrated to show anti-inflammatory activity in the intestinal mucosa. Notably, hydrolyzed diets combined with the administration of PRBs and GAGs offer a promising approach for the treatment of canine IBD. Our aim was to investigate the effects of hydrolyzed diet and GAG+PRB co-treatment on the serum metabolomic profile of IBD dogs. Dogs with IBD randomly received either hydrolyzed diet supplemented with GAGs and PRBs (treatment 1) or hydrolyzed diet alone (treatment 2) for 10 weeks. A targeted metabolomics approach using mass spectrometry was performed to quantify changes in the serum metabolome before and after treatment and between treatment 1 and 2. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), hierarchical cluster analysis (HCA) and univariate statistics were used to identify differences between the treatment groups. PCA, PLS-DA, and HCA showed a clear clustering of IBD dogs before and after hydrolyzed diet, indicating that the treatment impacted the serum metabolome. Univariate analysis revealed that most of the altered metabolites were involved in lipid metabolism. The most impacted lipid classes were components of cell membranes, including glycerophospholipids, sphingolipids, and di- and triglycerides. In addition, changes in serum metabolites after GAG+PRB co-treatment suggested a possible additional beneficial effect on the lipid metabolism in IBD dogs. In conclusion, the present study showed a significant increase in metabolites that protect gut cell membrane integrity in response to hydrolyzed diet alone or in combination with GAG+PRB co-treatment. Administration of such treatment over 70 days improved selected serum biomarkers of canine IBD, possibly indicating improved intestinal membrane integrity.

【 授权许可】

Unknown