Production and characterization of polyclonal antibody against Bengal tiger (Panthera tigris tigris) immunoglobulin G

Journal of Applied Animal Research

Ruksiri Nomsiri¹  Pinich Boontong²  Preeyanat Vongchan³  Rinrada Sangchantip⁴  Surapa Rungphattanachaikul⁴  Amarin Rittipornlertrak⁴  Itsarapan Fhaikruae⁴  Chanakan Areewong⁴  Waroot Wongkalasin⁴  Nattawooti Sthitmatee⁴ 

[1] Chiang Mai Night Safari;Chiang Mai Zoo;Faculty of Associated Medical Sciences, Department of Medical Technology, Chiang Mai University;Faculty of Veterinary Medicine, Chiang Mai University;

关键词: bengal tiger;    immunoglobulin g;    indirect elisa;    panthera tigris tigris;    polyclonal antibody;   

DOI  :  10.1080/09712119.2019.1629937

来源: DOAJ

【 摘 要 】

The immunoglobulin G (IgG) antibody is important for detecting and evaluating immune responses. Despite increasing opportunities for tigers to be exposed to emerging diseases due to global changes coupled with increasing interaction with humans, there is no available antibody specific to tigers. Aims of this study were to produce a rabbit anti-Bengal tiger (Panthera tigris tigris) IgG polyclonal antibody and to determine its specificity. The molecular weight of the purified Bengal tiger IgG was approximately 170 kDa. The cross-reactivity of rabbit anti-Bengal tiger IgG to IgG of other wild felid species and other domestic animals was analyzed by indirect enzyme-linked immunosorbent assay. The cross reactivity to other related wild animals ranged from 53.69% to 75.63%. There was high cross-reactivity with domestic cats (63.58%) and pigs (38.45%) and low cross-reactivity with domestic cows (10.46%), goats (8.60%), sheep (7.25%), and chickens (6.91%). To confirm cross-reactivity with domestic animals’ IgG, western blotting was performed. These results indicated that the purified rabbit anti-Bengal tiger IgG polyclonal antibody strongly reacted with species in the family Felidae. The polyclonal antibody generated in this study has the potential to aid in the development of useful tools for further novel immunological investigations on tigers and related felid species.

【 授权许可】

Unknown