Review of methods used for identification of biothreat agents in environmental protection and human health aspects

Annals of Agricultural and Environmental Medicine

PiotrCieślik¹  Aleksander J. Michalski¹  TomaszMirski¹  AgataBielawska-Drózd¹  MichałBartoszcze²  MarcinNiemcewicz²  KrzysztofChomiczewski³  JanuszKocik⁴ 

[1] Biological Threat Identification and Countermeasure Centre of the Military Institute of Hygiene and Epidemiology, Puławy, Poland ;Biological Threat Identification and Countermeasure Centre of the Military Institute of Hygiene and Epidemiology, Puławy, Poland ;Faculty of Chemistry and New Technologies, Military Uniwersity of Technology, Warsaw, Poland;Military Institute of Hygiene and Epidemiology, Department of Epidemiology, Warsaw, Poland ;

关键词: genetic methods;    immunological methods;    Bioterrorism;    diagnostic techniques;   

DOI  :  

来源: DOAJ

【 摘 要 】

Modern threats of bioterrorism force the need to develop methods for rapid and accuratedentification of dangerousbiological agents. Currently, there are many types of methods used in this field of studies that are based on immunologicalor genetic techniques, or constitute a combination of both methods (immuno-genetic). There are also methods that havebeen developed on the basis of physical and chemical properties of the analytes. Each group of these analytical assays canbe further divided into conventional methods (e.g. simple antigen-antibody reactions, classical PCR,eal-time PCR), andmodern technologies (e.g. microarray technology, aptamers, phosphors, etc.). Nanodiagnostics constitute another groupof methods that utilize the objects at a nanoscale (below 100 nm). There are also integrated and automated diagnosticsystems, which combine differentethods and allow simultaneous sampling, extraction of genetic material and detectionand identification of the analyte using genetic, as well as immunological techniques.

【 授权许可】

Unknown