Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster

Emerging Microbes and Infections

Víctor Guallar¹  Alfonso Valencia¹  Albert Bensaid²  Núria Roca²  Guillermo Cantero²  Mónica Pérez²  Marco Brustolin²  Jordi Rodon²  Nigeer Te²  Júlia Vergara-Alert²  Jorge Carrillo³  Nuria Izquierdo-Useros³  Julià Blanco³  María Luisa Rodríguez de la Concepción³  Carlos Ávila-Nieto³  Bonaventura Clotet³  Marc Noguera-Julián³  Joaquim Segalés⁴ 

[1] Barcelona Supercomputing Center (BSC), Barcelona, Spain;IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Caldes de Montbui, Spain;IrsiCaixa AIDS Research Institute, Badalona, Spain;UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Barcelona, Spain;

关键词: SARS-CoV-2;    golden Syrian hamster;    animal model;    infection;    re-infection;    protection;   

DOI  :  10.1080/22221751.2021.1913974

来源: DOAJ

【 摘 要 】

Reinfections with SARS-CoV-2 have already been documented in humans, although its real incidence is currently unknown. Besides having a great impact on public health, this phenomenon raises the question of immunity generated by a single infection is sufficient to provide sterilizing/protective immunity to a subsequent SARS-CoV-2 re-exposure. The Golden Syrian hamster is a manageable animal model to explore immunological mechanisms able to counteract COVID-19, as it recapitulates pathological aspects of mild to moderately affected patients. Here, we report that SARS-CoV-2-inoculated hamsters resolve infection in the upper and lower respiratory tracts within seven days upon inoculation with the Cat01 (G614) SARS-CoV-2 isolate. Three weeks after the primary challenge, and despite high titres of neutralizing antibodies, half of the animals were susceptible to reinfection by both identical (Cat01, G614) and variant (WA/1, D614) SARS-CoV-2 isolates. However, upon re-inoculation, only nasal tissues were transiently infected with much lower viral replication than those observed after the first inoculation. These data indicate that a primary SARS-CoV-2 infection is not sufficient to elicit a sterilizing immunity in hamster models but protects against lung disease.

【 授权许可】

Unknown