Structure and biological activity of particles produced from highly activated carbon adsorbent

Heliyon

Alexandra Lykhova¹  Alexey Sidorenko²  Anatolii Belous²  Veronika Sarnatskaya²  Igor Prokopenko²  Olga Brieieva²  Serhii Solopan²  Denis Kolesnik³  Yuliia Shlapa³  Vladimir Nikolaev³ 

[1] Corresponding author.;Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Ukraine;V. I. Vernadsky Institute of General and Inorganic Chemistry of the NAS of Ukraine, 32/34, Palladina Ave., Kyiv, 03142, Ukraine;

关键词: Carbon particles;    BSA conformation;    Cytotoxicity;    Genotoxicity;   

DOI  :  

来源: DOAJ

【 摘 要 】

Over the recent years, carbon particles have gained relevance in the field of biomedical application to diminish the level of endo-/exogenous intoxication and oxidative stress products, which occur at different pathological states. However, it is very important that such carbon particles, specially developed for parenteral administration or per oral usage, possess a high adsorption potential and can remove hazard toxic substances of the hydrophilic, hydrophobic and amphiphilic nature usually accumulated in the blood due to the disease, and be absolutely safe for normal living cells and tissues of organism. In this work, the stable monodisperse suspension containing very small-sized (Dhydro = 1125.3 ± 243.8 nm) and highly pure carbon particles with an excellent accepting ability were obtained. UV-spectra, fluorescence quenching constant and binding association constant were provided by the information about conformational alterations in an albumin molecule in presence of carbon particles, about the dynamic type of quenching process and low binding affinity between carbon and protein. The later was confirmed by DSC method. In vitro cell culture experiments showed that carbon particles did not possess any cytotoxic effect towards all testing the normal cell lines of different histogenesis, did not show genotoxic effects and were absolutely safe for experimental animals during and after their parenteral administration. These observations may provide more information about how to develop a safe preparation of carbon particles for different biomedical applications, in particular, as a mean for intracorporeal therapy of various heavy diseases accompanied by the increased endogenous intoxication and the level of oxidative stress.

【 授权许可】

Unknown