Haslea nusantara (Bacillariophyceae), a new blue diatom from the Java Sea, Indonesia: morphology, biometry and molecular characterization

Plant Ecology and Evolution

Michel Poulin¹  Charlotte Falaise²  Romain Gastineau²  Vincent Leignel²  Jean-Luc Mouget²  Fiddy Prasetiya²  Yann Hardivillier²  Ita Widowati³  Sulastri Arsad⁴  Yenny Risjani⁴  Agung Syakti⁵  Monique Turmel⁶  Claude Lemieux⁶  Iskandar Iskandar⁷  Ikha Safitri⁸  Toto Subroto⁹ 

[1] Division Recherche et collections, Musée canadien de la nature, C.P. 3443, Succursale D, Ottawa, Ontario, K1P 6P4;FR CNRS 3473 IUML, Mer-Molécules-Santé (MMS), Le Mans Université, Avenue Olivier Messiaen, 72085 Le Mans CEDEX 9;Faculty of Fisheries and Marine Sciences, Diponegoro University, Jl. Prof. H. Soedharto,SH, Tembalang, Semarang 50275;Faculty of Fisheries and Marine Sciences, University of Brawijaya, Jalan Veteran, Ketawanggede, Malang 65145;Fisheries and Marine Sciences Department, Jenderal Soedirman University, Kampus Perikanan Unsoed Karangwangkal, Jl dr. Suparno, Purwokerto 53123;Institut de Biologie Intégrative et des Systèmes, Département de Biochimie, de Microbiologie et de Bio-Informatique, Université Laval, Québec;Marine Science Department, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM.21, Jatinangor 45363;Marine Science Department, Faculty of Mathematics and Natural Sciences Universitas Tanjungpura, Jl. Prof. Dr. H. Hadari Nawawi, Pontianak, Kalimantan Barat 78124;Research Center of Biotechnology and Bioinformatics, Jl. Raya Bandung Sumedang KM.21, Jatinangor 45363;

关键词: Indonesia;    Bacillariophyceae;    blue diatoms;    Haslea;   

DOI  :  10.5091/plecevo.2019.1623

来源: DOAJ

【 摘 要 】

Background and aims – The present study aims to describe a new species of pennate blue diatom from the genus Haslea, H. nusantara sp. nov., collected from Semak Daun Island, the Seribu Archipelago, in Indonesian marine waters.Methods – Assessment for species identification was conducted using light microscopy, Scanning Electron Microscopy and molecular techniques. The morphological characteristics of H. nusantara have been described, illustrated and compared to other morphologically similar blue Haslea taxa, distributed worldwide. Additionally, molecular characterization was achieved by sequencing plastidial and mitochondrial genomes.Key results – This new species, named Haslea nusantara, cannot be discriminated by its morphology (stria density) but it is characterized by its gene sequences (rbcL chloroplast gene and cox1 mitochondrial gene). Moreover, it differentiates from other blue Haslea species by the presence of a thin central bar, which has been previously reported in non-blue species like H. pseudostrearia. The complete mitochondrion (36,288 basepairs, bp) and plastid (120,448 bp) genomes of H. nusantara were sequenced and the gene arrangements were compared with other diatom genomes. Phylogeny analyses established using rbcL indicated that H. nusantara is included in the blue Haslea cluster and close to a blue Haslea sp. found in Canary Islands (H. silbo sp. ined.).Conclusions – All investigations carried out in this study show that H. nusantara is a new blue-pigmented species, which belongs to the blue Haslea clade, with an exceptional geographic distribution in the Southern Hemisphere.

【 授权许可】

Unknown