Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Bioscience Journal

Gustavo Graciano Fonseca¹  Rodrigo Simões Ribeiro Leite²  Rodrigo Prudente Scalabrini²  Marcelo Fossa da Paz²  Ana Carolina da Costa²  Maria Alice Silvestre²  André Rodrigues³ 

[1] Laboratory of Bioengineering, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Rodovia Dourados/Itahum, km 12, CEP 79804-970, Dourados ;Laboratory of Enzymology and Fermentation Processes, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados (FCBA/UFGD), Rodovia Dourados/Itahum, km 12, CEP 79804-970, Dourados ;Laboratory of Fungal Ecology and Systematics, Biosciences Institute, Department of Biochemistry and Microbiology, São Paulo State University (IB/UNESP), Av. 24-A, n.1515, CEP 13506-900, Rio Claro ;

关键词: Solid State Fermentation;    Pulp and paper bleaching;    Cellulases and hemicellulases;   

DOI  :  

来源: DOAJ

【 摘 要 】

Xylanases are useful in several industrial segments, including pulp and paper bleaching, animal feed, and bread-making processes. However, the industrial use of these enzymes is closely related to its production cost and its catalytic properties. The process of solid state fermentation enables the use of agro-industrial residues as substrates for microbial cultivation and enzymes production, reducing costs. In the present study, different cultivation parameters were evaluated for the xylanase production by the thermophilic fungus Thermoascus aurantiacus, by solid state fermentation, using agro-industrial residues as substrates. High production of xylanase (1701.9 U g-1 of dry substrate) was obtained using wheat bran containing 65% of initial moisture, at 120 h of cultivation, and 45°C. The xylanase showed optimal activity at pH 5.0 and 75°C; its stability was maintained at pH 3.0-11.0. The enzyme retained its catalytic potential after 1 h, at 75°C. The enzymatic extract produced under optimized conditions showed reduced activities of endoglucanase and FPase. Our results, including the xylanase production by T. aurantiacus in low-cost cultivation medium, high structural stability of the enzyme, and reduced cellulolytic activity, encourage the application of this enzymatic extract in pulp and paper bleaching processes. 

【 授权许可】

Unknown