Loss of Motor Protein MYO1C Causes Rhodopsin Mislocalization and Results in Impaired Visual Function

Cells

AshishK. Solanki¹  Ehtesham Arif¹  Stephen Walterhouse¹  Bushra Rahman¹  GlennPrazere Lobo¹  SandraR. Montezuma²  AltafA. Kondkar³  Shahid Husain⁴  ManasR. Biswal⁵  René Martin⁶  Hans-Joachim Knölker⁶  Deepak Nihalani⁷ 

[1] Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA;Department of Ophthalmology and Visual Neurosciences, University of Minnesota, 516 Delaware Street S.E., 9th Floor, Minneapolis, MN 55455, USA;Department of Ophthalmology, College of Medicine, King Saud University, Riyadh 11411, Saudi Arabia;Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA;Department of Pharmaceutical Sciences, Taneja College of Pharmacy, University of South Florida, Tampa, FL 33612, USA;Faculty of Chemistry, Technische Universität Dresden, Bergstraße 66, 01069 Dresden, Germany;National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bldg. 2DEM, Room 6085, 6707 Democracy Blvd., Bethesda, MD 20817, USA;

关键词: motor protein;    myosin 1C;    photoreceptor;    rhodopsin;    retina;    outer segments;   

DOI  :  10.3390/cells10061322

来源: DOAJ

【 摘 要 】

Unconventional myosins, linked to deafness, are also proposed to play a role in retinal cell physiology. However, their direct role in photoreceptor function remains unclear. We demonstrate that systemic loss of the unconventional myosin MYO1C in mice, specifically causes rhodopsin mislocalization, leading to impaired visual function. Electroretinogram analysis of Myo1c knockout (Myo1c-KO) mice showed a progressive loss of photoreceptor function. Immunohistochemistry and binding assays demonstrated MYO1C localization to photoreceptor inner and outer segments (OS) and identified a direct interaction of rhodopsin with MYO1C. In Myo1c-KO retinas, rhodopsin mislocalized to rod inner segments (IS) and cell bodies, while cone opsins in OS showed punctate staining. In aged mice, the histological and ultrastructural examination of the phenotype of Myo1c-KO retinas showed progressively shorter photoreceptor OS. These results demonstrate that MYO1C is important for rhodopsin localization to the photoreceptor OS, and for normal visual function.

【 授权许可】

Unknown