Extract of Monascus purpureus CWT715 Fermented from Sorghum Liquor Biowaste Inhibits Migration and Invasion of SK-Hep-1 Human Hepatocarcinoma Cells

Molecules

Wen-Teish Chang¹  Chin-Shiu Huang²  Wan-Ju Lee²  Cheng-Hung Chuang³ 

[1] Department of Food Science, National Penghu University of Science and Technology, Penghu Hsien 88046, Taiwan;Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan;Department of Nutrition, Hungkuang University, 1018 Sec. 6 Taiwan Boulevard, Taichung 43302, Taiwan;

关键词: Kinmen sorghum liquor waste;    Monascus purpureus;    anti-metastasis;    nm23-H1;    hepatocarcinoma;   

DOI  :  10.3390/molecules21121691

来源: DOAJ

【 摘 要 】

Liver cancer is the most endemic cancer in a large region of the world. This study investigated the anti-metastatic effects of an extract of Monascus purpureus CWT715 (MP) fermented from sorghum liquor biowaste and its mechanisms of action in highly metastatic human hepatocarcinoma SK-Hep-1 cells. Kinmen sorghum liquor waste was used as the primary nutrient source to produce metabolites (including pigments) of MP. In the presence of 10 µg/mL MP-fermented broth (MFB), the anti-invasive activity increased with increasing fermentation time reaching a maximum at six days of fermentation. Interestingly, MFB also produced maximal pigment content at six days. Treatment for 24 h with MFB (10–100 µg/mL) obtained from fermentation for six days significantly inhibited cell migration and invasion, and these effects were concentration-dependent. MFB also significantly enhanced nm23-H1 protein expression in a concentration-dependent manner, which was highly correlated with migration and invasion. These results suggest that MFB has significant anti-migration and anti-invasion activities and that these effects are associated with the induction of nm23-H1 protein expression.

【 授权许可】

Unknown