Molecular Characterization of Donacia provosti (Coleoptera: Chrysomelidae) Larval Transcriptome by De Novo Assembly to Discover Genes Associated with Underwater Environmental Adaptations

Insects

Cheng Qu¹  Fengqi Li¹  Haixia Zhan¹  Chen Luo¹  Youssef Dewer²  Shiyong Yang³  Liangjun Li⁴ 

[1] Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;Central Agricultural Pesticide Laboratory, Agricultural Research Center, Bioassay Research Department, Dokki, Giza 12618, Egypt;School of Ecology and Environment, Anhui Normal University, Wuhu 241000, China;School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China;

关键词: Donacia provosti;    aquatic lifestyle;    transcriptome assembly;    positive selection;    adaptive evolution;   

DOI  :  10.3390/insects12040281

来源: DOAJ

【 摘 要 】

Donacia provosti (Fairmaire, 1885) is a major pest of aquatic crops. It has been widely distributed in the world causing extensive damage to lotus and rice plants. Changes in gene regulation may play an important role in adaptive evolution, particularly during adaptation to feeding and living habits. However, little is known about the evolution and molecular mechanisms underlying the adaptation of D. provosti to its lifestyle and living habits. To address this question, we generated the first larval transcriptome of D. provosti. A total of 20,692 unigenes were annotated from the seven public databases and around 18,536 protein-coding genes have been predicted from the analysis of D. provosti transcriptome. About 5036 orthologous cutlers were identified among four species and 494 unique clusters were identified from D. provosti larvae including the visual perception. Furthermore, to reveal the molecular difference between D. provosti and the Colorado potato beetle Leptinotarsa decemlineata, a comparison between CDS of the two beetles was conducted and 6627 orthologous gene pairs were identified. Based on the ratio of nonsynonymous and synonymous substitutions, 93 orthologous gene pairs were found evolving under positive selection. Interestingly, our results also show that there are 4 orthologous gene pairs of the 93 gene pairs were associated with the “mTOR signaling pathway”, which are predicted to be involved in the molecular mechanism of D. provosti adaptation to the underwater environment. This study will provide us with an important scientific basis for building effective prevention and control system of the aquatic leaf beetle Donacia provosti.

【 授权许可】

Unknown