Resveratrol attenuated hydrogen peroxide-induced myocardial apoptosis by autophagic flux

Food & Nutrition Research

Wan-Teng Lin¹  Jing-Yi Yang¹  Wei-Jen Ting³  Chih-Yang Huang⁴ 

[1] Department of Hospitality Management, College of Agriculture, Tunghai University, Taichung, Taiwan;Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan;School of Chinese Medicine, China Medical University, Taichung, Taiwan;Translation Research Core, China Medical University Hospital, Taichung, Taiwan;

关键词: resveratrol;    oxidative stress;    apoptosis;    autophagy;   

DOI  :  10.3402/fnr.v60.30511

来源: DOAJ

【 摘 要 】

Background: Resveratrol is a Sirt-1-specific activator, which also exerts cardioprotective effects that regulate redox signalling during oxidative stress and autophagy during cardiovascular disease (CVD). Objective: This study investigated the protective effects of resveratrol against hydrogen peroxide-induced damage in cardiomyocytes. Design: In this article, hydrogen peroxide-induced autophagy and apoptosis in H9c2 cardiomyoblasts were studied at an increasing concentration from 0 to 100 µM. Results: Resveratrol pretreatment with concentrations of 10, 20, and 50 µM inhibits autophagic apoptosis by increasing p-Akt and Bcl-2 protein levels in H9c2 cells. Interestingly, resveratrol treatment activates the Beclin-1, LC3, p62, and the lysosome-associated protein LAMP2a within 24 h of administration. Conclusions: These results suggest that resveratrol-regulated autophagy may play a role in degrading damaged organelles in H9c2 cells rather than causing apoptosis, and this may be a possible mechanism by which resveratrol protects the heart during CVD.

【 授权许可】

Unknown