A Similar Secretome Disturbance as a Hallmark of Non-pathogenic Botrytis cinerea ATMT-Mutants?

Frontiers in Microbiology

François Villalba¹  Marie-Pascale Latorse¹  Lucie Chandat¹  Julia Schumacher²  Cindy Dieryckx³  Sophie Kaiser³  Nathalie Poussereau³  Elise Loisel³  Christine Rascle³  Christophe Bruel³  Mathias Choquer³  Amélie de Vallée³  Eytham Souibgui³  Géraldine Mey³  Jean William Dupuy⁴  Guillaume Morgant⁵  Adeline Simon⁵  Muriel Viaud⁵  Pascal Bally⁵ 

[1] Centre de Recherche La Dargoire, Bayer SAS, Lyon, France;Federal Institute for Materials Research and Testing (BAM), Berlin, Germany;Microbiologie, Adaptation et Pathogénie, UMR 5240, Univ Lyon, Université Lyon 1, Bayer SAS, Lyon, France;Plateforme Protéome, Centre de Génomique Fonctionnelle, Université de Bordeaux, Bordeaux, France;UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, Thiverval-Grignon, France;

关键词: Botrytis cinerea;    Agrobacterium tumefaciens-mediated transformation;    pathogenicity;    secretomics;    transcriptomics;   

DOI  :  10.3389/fmicb.2019.02829

来源: DOAJ

【 摘 要 】

The gray mold fungus Botrytis cinerea is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to decipher its infectious strategy, a library of 2,144 mutants was generated by random insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation (ATMT). Twelve mutants exhibiting total loss of virulence toward different host plants were chosen for detailed analyses. Their molecular characterization revealed a single T-DNA insertion in different loci. Using a proteomics approach, the secretome of four of these strains was compared to that of the parental strain and a common profile of reduced lytic enzymes was recorded. Significant variations in this profile, notably deficiencies in the secretion of proteases and hemicellulases, were observed and validated by biochemical tests. They were also a hallmark of the remaining eight non-pathogenic strains, suggesting the importance of these secreted proteins in the infection process. In the twelve non-pathogenic mutants, the differentiation of infection cushions was also impaired, suggesting a link between the penetration structures and the secretion of proteins involved in the virulence of the pathogen.

【 授权许可】

Unknown