Raman Spectroscopy of Individual Cervical Exfoliated Cells in Premalignant and Malignant Lesions

Applied Sciences

César Arturo Ilizaliturri Hernández¹  Mariana Sarai Silva-López²  Ángel Gabriel Rodríguez Vázquez²  Hugo Ricardo Navarro Contreras²  Eleazar Samuel Kolosovas-Machuca²  Alejandra Ortiz-Dosal³ 

[1] CIACYT-Facultad de Medicina, Universidad Autónoma de San Luís Potosí, 550-2a Sierra Leona Ave, San Luis Potosí 78210, Mexico;Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, 550-2a Sierra Leona Ave, San Luis Potosí 78210, Mexico;Doctorado Institucional en Ingeniería y Ciencia de Materiales (DICIM-UASLP), Universidad Autónoma de San Luis Potosí, 550 Sierra Leona Ave, San Luis Potosí 78210, Mexico;

关键词: cancer screening;    cervical cytology;    single-cell Raman spectroscopy;    cervical intraepithelial neoplasia;    malignant cervical lesions;   

DOI  :  10.3390/app12052419

来源: DOAJ

【 摘 要 】

Cervical cancer is frequent neoplasia. Currently, the diagnostic approach includes cervical cytology, colposcopy, and histopathology studies; combining detection techniques increases the sensitivity and specificity of the tests. Raman spectroscopy is a high-resolution technique that supports the diagnosis of malignancies. This study aimed to evaluate the Raman spectroscopy technique discriminating between healthy and premalignant/malignant cervical cells. We included 81 exfoliative cytology samples, 29 in the “healthy group” (negative cytology), and 52 in the “CIN group” (premalignant/malignant lesions). We obtained the nucleus and cytoplasm Raman spectra of individual cells. We tested the spectral differences between groups using Permutational Multivariate Analysis of Variance (PERMANOVA) and Canonical Analysis of Principal Coordinates (CAP). We found that Raman spectra have increased intensity in premalignant/malignant cells compared with healthy cells. The characteristic Raman bands corresponded to proteins and nucleic acids, in concordance with the increased replication and translation processes in premalignant/malignant states. We found a classification efficiency of 76.5% and 82.7% for cytoplasmic and nuclear Raman spectra, respectively; cell nucleus Raman spectra showed a sensitivity of 84.6% in identifying cervical anomalies. The classification efficiency and sensitivity obtained for nuclear spectra suggest that Raman spectroscopy could be helpful in the screening and diagnosis of premalignant lesions and cervical cancer.

【 授权许可】

Unknown