Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity

eLife

Erika K Williams¹  Stephen D Liberles²  Victor Wang²  Seungkyu Lee²  David P Roberson²  Cheryl L Stucky²  Lee B Barrett²  Cara Piccoli³  Andy D Weyer⁴  Shan Lou⁴  Nader Ghasemlou⁵  Isaac M Chiu⁶  Qiufu Ma⁶  Clifford J Woolf⁶  Ezgi Ahat⁶  Gregory S Bryman⁶  David E Strochlic⁶  Enrique J Cobos⁶ 

[1] Department of Microbiology and Immunobiology, Harvard Medical School, Boston, United States;Department of Neurobiology, Harvard Medical School, Boston, United States;Dana-Farber Cancer Institute, Harvard Medical School, Boston, United States;Department of Cell Biology, Harvard Medical School, Boston, United States;Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, United States;F.M. Kirby Neurobiology Center, Boston Children's Hospital, Boston, United States;

关键词: transcriptome;    peripheral nervous system;    somatosensation;    DRG;    nociception;    proprioception;   

DOI  :  10.7554/eLife.04660

来源: DOAJ

【 摘 要 】

The somatosensory nervous system is critical for the organism's ability to respond to mechanical, thermal, and nociceptive stimuli. Somatosensory neurons are functionally and anatomically diverse but their molecular profiles are not well-defined. Here, we used transcriptional profiling to analyze the detailed molecular signatures of dorsal root ganglion (DRG) sensory neurons. We used two mouse reporter lines and surface IB4 labeling to purify three major non-overlapping classes of neurons: 1) IB4+SNS-Cre/TdTomato+, 2) IB4−SNS-Cre/TdTomato+, and 3) Parv-Cre/TdTomato+ cells, encompassing the majority of nociceptive, pruriceptive, and proprioceptive neurons. These neurons displayed distinct expression patterns of ion channels, transcription factors, and GPCRs. Highly parallel qRT-PCR analysis of 334 single neurons selected by membership of the three populations demonstrated further diversity, with unbiased clustering analysis identifying six distinct subgroups. These data significantly increase our knowledge of the molecular identities of known DRG populations and uncover potentially novel subsets, revealing the complexity and diversity of those neurons underlying somatosensation.

【 授权许可】

Unknown