A Mouse Model of Oropharyngeal Papillomavirus-Induced Neoplasia Using Novel Tools for Infection and Nasal Anesthesia

Viruses

RonnieW. AlRamahi¹  JoshP. Schroeder¹  JaredT. Piette¹  LouisA. Hinshaw¹  AndrewD. Kurth¹  KristynS. Masters¹  Darya Buehler²  SusanL. Thibeault³  ReneeE. King³  Andrea Bilger⁴  MatthewV. Barthel⁴  EllaT. Ward-Shaw⁴  PaulF. Lambert⁴ 

[1] Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53705, USA;Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53705, USA;Department of Surgery, University of Wisconsin, Madison, WI 53705, USA;McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI 53705, USA;

关键词: mouse;    papillomavirus;    throat;    head-and-neck;    OPSCC;    oropharynx;   

DOI  :  10.3390/v12040450

来源: DOAJ

【 摘 要 】

Human head and neck cancers that develop from the squamous cells of the oropharynx (Oropharyngeal Squamous Cell Carcinomas or OPSCC) are commonly associated with the papillomavirus infection. A papillomavirus infection-based mouse model of oropharyngeal tumorigenesis would be valuable for studying the development and treatment of these tumors. We have developed an efficient system using the mouse papillomavirus (MmuPV1) to generate dysplastic oropharyngeal lesions, including tumors, in the soft palate and the base of the tongue of two immune-deficient strains of mice. To maximize efficiency and safety during infection and endoscopy, we have designed a nose cone for isoflurane-induced anesthesia that takes advantage of a mouse’s need to breathe nasally and has a large window for oral manipulations. To reach and infect the oropharynx efficiently, we have repurposed the Greer Pick allergy testing device as a virus delivery tool. We show that the Pick can be used to infect the epithelium of the soft palate and the base of the tongue of mice directly, without prior scarification. The ability to induce and track oropharyngeal papillomavirus-induced tumors in the mouse, easily and robustly, will facilitate the study of oropharyngeal tumorigenesis and potential treatments.

【 授权许可】

Unknown