Viability of bull spermatozoa collected from the epididymis stored at 18-20°C

Brazilian Archives of Biology and Technology

Aline Silva Fujita¹  Luiz Ernandes Kozicki²  Kerriel Thandile Green³  Melina Andrea Formighieri Bertol⁴  Vanete Thomaz-Soccol⁴  Renata Azevedo de Abreu⁴  Romildo Romualdo Weiss⁴ 

[1] ;Pontifícia Universidade Católica do Paraná;Universidade Estadual de Londrina;Universidade Federal do Paraná;

关键词: Bull;    Epididymis;    Orchiectomy;    Sperm;    Room temperature;    Viability;   

DOI  :  10.1590/S1516-89132013000500008

来源: DOAJ

【 摘 要 】

The aim of this study was to evaluate the viability of bull spermatozoa collected from the cauda epididymis stored at 18-20°C, which were compared with semen collected by electro-ejaculation method and preserved at 5°C. Ten pairs of testes from Tabapuã bulls were removed by orchiectomy and stored for 6 (G6), 12 (G12), 18 (G18), 24 (G24) and 30 (G30) h at room temperature (18-20°C). Seven days before orchiectomy, semen was collected by electro-ejaculation method. The sperm parameters evaluated were: sperm motility, vigor, concentration, morphology and acrosome defects. Sperm motility declined (p<0.05) when spermatozoa were stored for 30 h in the epididymis. The spermatozoa from the epididymis showed lower sperm motility than that of spermatozoa collected via electro-ejaculation. There was a little expressive decrease in sperm vigor and increased in morphological defects with storage time, but the acrosome integrity was not affected. Cold storage (5°C) maintained sperm viable for 15 to 40.8 h. Thus, it was possible to recover viable sperm with 41.25% of motility from the cauda epididymis stored at room temperature of 18-20°C for 30 h. There were differences between the ejaculated and epididymal sperm for the bulls and the conservation at 5°C allowed short-term preservation of the gametes.

【 授权许可】

Unknown