Human Cardiac Mesenchymal Stromal Cells From Right and Left Ventricles Display Differences in Number, Function, and Transcriptomic Profile

Frontiers in Physiology

Barbara Micheli¹  Anna Guarino¹  Gianluca Polvani¹  Chiara Vavassori²  Giulio Pompilio²  Luca Piacentini³  Mattia Chiesa³  Gualtiero Ivanoe Colombo³  Elena Sommariva⁴  Alessandro Scopece⁴  Ilaria Stadiotti⁴ 

[1] Cardiovascular Tissue Bank, Centro Cardiologico Monzino IRCCS, Milan, Italy;Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy;Unit of Immunology and Functional Genomics, Centro Cardiologico Monzino IRCCS, Milan, Italy;Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino IRCCS, Milan, Italy;

关键词: cardiac mesenchymal stromal cells;    cardiac ventricles;    functional studies;    transcriptome;    left ventricle;    right ventricle;   

DOI  :  10.3389/fphys.2020.00604

来源: DOAJ

【 摘 要 】

BackgroundLeft ventricle (LV) and right ventricle (RV) are characterized by well-known physiological differences, mainly related to their different embryological origin, hemodynamic environment, function, structure, and cellular composition. Nevertheless, scarce information is available about cellular peculiarities between left and right ventricular chambers in physiological and pathological contexts. Cardiac mesenchymal stromal cells (C-MSC) are key cells affecting many functions of the heart. Differential features that distinguish LV from RV C-MSC are still underappreciated.AimTo analyze the physiological differential amount, function, and transcriptome of human C-MSC in LV versus (vs.) RV.MethodsHuman cardiac specimens of LV and RV from healthy donors were used for tissue analysis of C-MSC number, and for C-MSC isolation. Paired LV and RV C-MSC were compared as for surface marker expression, cell proliferation/death ratio, migration, differentiation capabilities, and transcriptome profile.ResultsHistological analysis showed a greater percentage of C-MSC in RV vs. LV tissue. Moreover, a higher C-MSC amount was obtained from RV than from LV after isolation procedures. LV and RV C-MSC are characterized by a similar proportion of surface markers. Functional studies revealed comparable cell growth curves in cells from both ventricles. Conversely, LV C-MSC displayed a higher apoptosis rate and RV C-MSC were characterized by a higher migration speed and collagen deposition. Consistently, transcriptome analysis showed that genes related to apoptosis regulation or extracellular matrix organization and integrins were over-expressed in LV and RV, respectively. Besides, we revealed additional pathways specifically associated with LV or RV C-MSC, including energy metabolism, inflammatory response, cardiac conduction, and pluripotency.ConclusionTaken together, these results contribute to the functional characterization of RV and LV C-MSC in physiological conditions. This information suggests a possible differential role of the stromal compartment in chamber-specific pathologic scenarios.

【 授权许可】

Unknown