Clearance of carbonyl-modified lipoproteins from the bloodstream of rabbits

Acta Biomedica Scientifica

V. Z. Lankin¹  G. G. Konovalova¹  S. P. Domogatskiy¹  A. K. Tikhaze¹  M. A. Grechnikova¹ 

[1] Russian Cardiology Research and Production Complex;

关键词: oxidative stress;    modified ldl;    mda;    glyoxal;    methylglyoxal;   

DOI  :  10.12737/article_590823a4b59f98.13160142

来源: DOAJ

【 摘 要 】

We have suggested that the molecular mechanism of vascular wall damage in diabetes is not substantially different from that in atherosclerosis. Thus, it can be assumed that aldehyde-modified LDL should be eliminated from the blood stream with much greater speed than non-oxidized LDL. In the available literature there is information about the clearance of native human LDL from the bloodstream, whereas information on the clearance of the aldehyde-modified LDL in animals or humans was not found. Based on this, the present work is devoted to the clearance of aldehyde-modified LDL of rabbits and humans introduced into the bloodstream of rabbits. We investigated the clearance of glyoxal-, methylglyoxal- and MDA-modified LDL from the bloodstream of rabbits. We used biotinylated LDL of rabbit blood plasma and FITC-labeled LDL of human blood plasma. LDL was isolated with preparative ultracentrifugation in NaBr gradient. It was shown that glyoxal- and methylglyoxal-modified LDL of rabbits and humans circulated in the bloodstream for the same time as native LDL while MDA-modified LDL was rapidly eliminated from the bloodstream. The data obtained indicated the possibility of greater atherogenic potential of glyoxal- and methylglyoxal-modified LDL as they circulate in the bloodstream for a rather long time. At the same time, MDA-modified LDL is likely to be exposed to enhanced elimination by macrophages after their "linkage" to blood cells.

【 授权许可】

Unknown