Improvements in Gene Editing Technology Boost Its Applications in Livestock

Frontiers in Genetics

Zhiqiang Fan¹  Irina A. Polejaeva¹  Iuri Viotti Perisse¹  Kenneth L. White¹  Galina N. Singina² 

[1] Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States;L.K. Ernst Federal Research Center for Animal Husbandry, Podolsk, Russia;

关键词: CRISPR/Cas9;    agriculture;    animal models;    livestock;    gene editing;   

DOI  :  10.3389/fgene.2020.614688

来源: DOAJ

【 摘 要 】

Accelerated development of novel CRISPR/Cas9-based genome editing techniques provides a feasible approach to introduce a variety of precise modifications in the mammalian genome, including introduction of multiple edits simultaneously, efficient insertion of long DNA sequences into specific targeted loci as well as performing nucleotide transitions and transversions. Thus, the CRISPR/Cas9 tool has become the method of choice for introducing genome alterations in livestock species. The list of new CRISPR/Cas9-based genome editing tools is constantly expanding. Here, we discuss the methods developed to improve efficiency and specificity of gene editing tools as well as approaches that can be employed for gene regulation, base editing, and epigenetic modifications. Additionally, advantages and disadvantages of two primary methods used for the production of gene-edited farm animals: somatic cell nuclear transfer (SCNT or cloning) and zygote manipulations will be discussed. Furthermore, we will review agricultural and biomedical applications of gene editing technology.

【 授权许可】

Unknown