The Onset of Systemic Oxidative Stress Associated with the Accumulation of Lipid Peroxidation Product Acrolein in the Skin of Patients with Small-Vessel Vasculitis

Molecules

Stela Bulimbasic¹  DanicaGalesic Ljubanovic²  Josko Mitrovic³  Jadranka Morovic-Vergles³  Kresimir Galesic⁴  Morana Jaganjac⁵  Neven Zarkovic⁵  Koji Uchida⁶  Franz Tatzber⁷  Vesna Sredoja Tisma⁸ 

[1] Department of Pathology and Cytology, University Hospital Centre Zagreb, 10000 Zagreb, Croatia;Department of Pathology, University of Zagreb, School of Medicine Zagreb, 10000 Zagreb, Croatia;Division of Clinical Immunology, Allergology and Rheumatology, Department of Internal Medicine, Dubrava University Hospital, University of Zagreb School of Medicine, 10040 Zagreb, Croatia;Division of Nephrology, Department of Internal Medicine, Dubrava University Hospital, University of Zagreb School of Medicine, 10040 Zagreb, Croatia;Laboratory for Oxidative Stress, Division of Molecular Medicine Rudjer Boskovic Institute, 10000 Zagreb, Croatia;Laboratory of Food and Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan;Omnignostica Gmbh & Co. KG, 2465 Höflein, Austria;Polyclinic Department of Dermatology and Venereology, Dubrava University Hospital, 10040 Zagreb, Croatia;

关键词: vasculitis;    oxidative stress;    lipid peroxidation;    acrolein;   

DOI  :  10.3390/molecules26082344

来源: DOAJ

【 摘 要 】

Small-vessel vasculitis (SVV) is the inflammation of the vessel wall that can result in hemorrhage and/or ischemia. Among the histological findings in SVV are increased infiltrating neutrophils, which, due to their oxidative burst and myeloperoxidase activity, release excessive reactive oxygen species, triggering a chain reaction of lipid peroxidation and yielding reactive aldehydes such as acrolein. The implication of oxidative stress in the pathogenesis of SVV was studied, focusing on acrolein immunohistochemistry in the affected skin vessels and systemic stress response. Samples from SVV patients and healthy subjects were collected and analyzed for total serum peroxides, total antioxidant capacity, inflammatory and immunological parameters, as well as for the presence of acrolein–protein adducts in the skin tissue specimens. The obtained data showed that systemic redox homeostasis and iron metabolism are altered in SVV patients. Possible biomarkers in the evaluation of oxidative status, disease activity and prevalence were indicated. Furthermore, a strong correlation between the accumulation of acrolein–protein adducts in the skin and the progression of the disease was revealed. Thus, the results of this study demonstrate that SVV is not only associated with systemic oxidative stress but also with tissue-specific oxidative stress that promotes acrolein formation and protein modification correlating with the severity of cutaneous vasculitis.

【 授权许可】

Unknown