RNAi efficiency, systemic properties and novel delivery methods for pest insect control: what we know so far.

Frontiers in Physiology

Moises João Zotti¹  Mallikarjuna Reddy Joga²  Guy Smagghe²  Olivier Christiaens² 

[1] Federal University of Pelotas;Ghent University;

关键词: Pest Control;    RNA interference (RNAi);    systemic RNAi;    DsRNA uptake;    DsRNA delivery;   

DOI  :  10.3389/fphys.2016.00553

来源: DOAJ

【 摘 要 】

In recent years, the research on the potential of using RNA interference (RNAi) to suppress crop pests has made an outstanding growth. However, given the variability of RNAi efficiency that is observed in many insects, the development of novel approaches towards insect pest management using RNAi requires first to unravel factors behind the efficiency of dsRNA-mediated gene silencing. In this review, we explore essential implications and possibilities to increase RNAi efficiency by delivery of dsRNA through non-transformative methods. We discuss factors influencing the RNAi mechanism in insects and systemic properties of dsRNA. Finally, novel strategies to deliver dsRNA are discussed, including delivery by symbionts, plant viruses, trunk injections, root soaking, and transplastomic plants.

【 授权许可】

Unknown