Visualizing evolution in real-time method for strain engineering

Frontiers in Microbiology

James eWinkler¹  Katy C. Kao²  Luis H Reyes² 

[1] M University;;Texas A&

关键词: Population Dynamics;    adaptive evolution;    strain development;    evolutionary engineering;   

DOI  :  10.3389/fmicb.2012.00198

来源: DOAJ

【 摘 要 】

The adaptive landscape for an industrially-relevant phenotype is determined by the effects of the genetic determinants on the fitness of the microbial system.Identifying the underlying adaptive landscape for a particular phenotype of interest will greatly enhance our abilities to engineer more robust microbial strains.Visualizing evolution in real-time (VERT) is a recently developed method based on in vitro adaptive evolution that facilitates the identification of fitter mutants throughout the course of evolution.Combined with high-throughput genomic tools, VERT can greatly enhance the mapping of adaptive landscapes of industrially-relevant phenotypes in microbial systems, thereby expanding our knowledge on the parameters that can be used for strain engineering.

【 授权许可】

Unknown