Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy

International Journal of Molecular Sciences

Stefano Leporatti¹  MariadM Vivanco²  Rafael Benítez³  Jagoba Iturri⁴  Alberto Moreno-Cencerrado⁴  Andreas Weber⁴  JoséLuis Toca-Herrera⁴ 

[1]CNR Nanotec-Istituto di Nanotecnologia, Polo di Nanotecnologia c/o Campus Ecoteckne, Via Monteroni, 73100 Lecce, Italy

[2]Cancer Heterogeneity Lab, CIC bioGUNE, Bizkaia Science and Technology Park, 48160 Derio, Spain

[3]Department Matemáticas para la Economía y la Empresa, Facultad de Economía, Universidad de Valencia, Avda. Tarongers s/n, 46022 Valencia, Spain

[4]Institute for Biophysics, Department of Nanobiotechnology (DNBT), BOKU University for Natural Resources and Life Sciences, Muthgasse 11 (Simon Zeisel Haus), A-1190 Vienna, Austria

关键词: resveratrol;    MCF-7;    cytomechanics;    atomic force microscopy;    force spectroscopy;    fluorescence microscopy;   

DOI  :  10.3390/ijms20133275

来源: DOAJ

【 摘 要 】

Atomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 µM) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized to determine simultaneous variations of Young’s modulus, the maximum adhesion force, and tether formation, thereby determining cell motility and adhesiveness. Effects of treatment were measured at several time-points (0−6 h, 24 h, and 48 h); longer exposures resulted in cell death. Our results demonstrated that AFM can be efficiently used as a diagnostic tool to monitor irreversible morpho/nano-mechanical changes in cancer cells during the early steps of drug treatment.

【 授权许可】

Unknown