SIRT1-SIRT7 Expression in Patients with Lymphoproliferative Disorders Undergoing Hematopoietic Stem Cell Mobilization

Cancers

Dominika Kulczycka-Wojdala¹  Emilia Stec-Martyna¹  Mateusz Nowicki²  Agnieszka Wierzbowska²  Anna Szmigielska-Kapłon²  Grzegorz Nowicki³ 

[1] Central Scientific Laboratory, Medical University of Lodz, 93-513 Lodz, Poland;Department of Hematology, Copernicus Memorial Hospital in Lodz Comprehensive Cancer Center and Traumatology, 93-513 Lodz, Poland;School of Continuing Studies, Georgetown University, Washington, DC 20001, USA;

关键词: sirtuins;    multiple myeloma;    lymphoma;    hematopoietic stem cells;    mobilization;    autologous transplantation;   

DOI  :  10.3390/cancers14051213

来源: DOAJ

【 摘 要 】

Sirtuins are involved in the fate of hematopoietic stem cells (HSCs), including their metabolism, stress response, differentiation, migration, and apoptosis. The aim of this study was to explore SIRT1-7 expression during HSC mobilization. The study included 50 patients with lymphoproliferative disorders (39 multiple myeloma, 11 lymphoma). Samples were taken before mobilization (day 0) and on the day of first apheresis (day A). The sirtuin expression was evaluated by the Droplet Digital PCR (ddPCR) method. A significant increase of the SIRT1, SIRT2, SIRT3, SIRT5, SIRT6, and SIRT7 levels measured at day A as compared to baseline was observed. The study revealed a positive correlation between SIRT5, SIRT6, and SIRT7 expression and the CD34+ peak value in peripheral blood and the number of CD34+ cells collected on day A. Patients from the SIRT7 “high expressors” group collected more CD34+ cells on day A than “low expressors”. Upregulated expressions of SIRT3 and SIRT7 on the day of first apheresis were observed in patients in complete remission status (CR) as compared to the non-CR group. Our results suggest that the investigated sirtuins may influence the HSC migration and hematopoietic landscape during mobilization. SIRT5, SIRT6, and SIRT7 may be associated with the efficacy of HSC mobilization.

【 授权许可】

Unknown