Critical reevaluation of the 4-(dimethylamino)cinnamaldehyde assay: Cranberry proanthocyanidin standard is superior to procyanidin A2 dimer for accurate quantification of proanthocyanidins in cranberry products

Journal of Functional Foods

Xin Chen¹  Nathan Chesmore²  Christian G. Krueger³  Jessica Parker³  Peter Crump⁴  Dhanansayan Shanmuganayagam⁴  Jannie P.J. Marais⁵  Jess D. Reed⁵  Christina Khoo⁵ 

[1] Corresponding author. Complete Phytochemical Solutions LLC, 317 South Street, Cambridge, WI 53523, USA. Tel.: [phone].;Reed Research Group, Dept. Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, WI 53706, USA;Complete Phytochemical Solutions LLC, 317 South Street, Cambridge, WI 53523, USA;Ocean Spray Cranberries Inc., One Ocean Spray Drive, Lakeville-Midddleboro, MA 02349, USA;Reed Research Group, Dept. Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, WI 53706, USA;

关键词: Proanthocyanidins;    Cranberry (Vaccinium macrocarpon Ait);    4- (dimethylamino)cinnamaldehyde;    DMAC;    MALDI-TOF MS;   

DOI  :  

来源: DOAJ

【 摘 要 】

The 4-(dimethylamino)cinnamaldehyde (DMAC) assay is currently used to quantify proanthocyanidin (PAC) content in cranberry products. In a multi-operator/multi-day study design, a cranberry proanthocyanidin (c-PAC) standard was compared to procyanidin A2 (ProA2) dimer for accurate quantification of PAC in commercial cranberry juices, lab generated cranberry blends and cranberry powders. The c-PAC standard reflects the structural heterogeneity of cranberry PAC degree of polymerization, hydroxylation pattern and ratios of ‘A-type’ to ‘B-type’ interflavanyl bonds. Use of the c-PAC standard to quantify PAC content in cranberry samples resulted in values that were 3.6 times higher than those determined by ProA2. Overall, there was no effect (P > 0.05) of operator or day on estimation of PAC concentration. The adoption of c-PAC standard should be considered as an improvement over the use of ProA2 for accurate quantification of cranberry PAC. Improved standardization of bioactive PAC components in functional cranberry foods will aid in establishment of dosage guidelines.

【 授权许可】

Unknown