Influence of climatic conditions on in vitro production of bovine embryos

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

R.B Navarro¹  K.E Brass¹  L.P Rauber¹  C.A.M Silva¹  M.I.B Rubin¹  G.A Pessoa¹ 

[1] Universidade Federal de Santa Maria;

关键词: bovino;    ovócitos;    embriões;    clima;    produção in vitro;   

DOI  :  10.1590/S0102-09352010000600013

来源: DOAJ

【 摘 要 】

Temperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus taurus semen in one incubator with 5% CO2. Presumable zygotes were cultured in gasified plastic bags with 5% CO2, 5% O2, and 90% N2. In the first year, cleavage and embryo yield were 60.3% and 15.6%, respectively, being lower (P<0.05) than in the following years. Average cleavage rates were always lower in winter (P<0.0001), thus producing less embryos. Winter climatic conditions had a negative influence on in vitro production, when cleavage and embryo yield declined, possibly because of reduced availability and growth of native pasture.

【 授权许可】

Unknown