Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells

Journal of King Saud University: Science

Govindan Ramachandran¹  Jia-Ling Li²  Shine Kadaikunnan³  Naiyf S. Alharbi³  Jamal M. Khaled⁴  Wen-Jun Li⁴  Lingzi Yin⁵  Arockiam Antony Joseph Velanganni⁵  Govindan Nadar Rajivgandhi⁶  Natesan Manoharan⁶  Moorthy Rajesh Kannan⁶ 

[1] Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India;State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, PR China;Department of Biochemistry, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India;State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China;

关键词: Actinomycetes;    Thin layer chromatography;    Preparative HPLC;    Cytotoxicity assay;    Morphological damages;    Nuclear damages;   

DOI  :  

来源: DOAJ

【 摘 要 】

The current study was concentrated to detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells. The partial purification of TLC was moved three different active spots. Among the spots, the third spot of the extract was shown excellent anti-cancer properties against MCF-7 human breast cancer cells. The available anti-cancer compounds present in the partially purified extract was confirmed by GC–MS data interpretation. Three fractions of the compounds were purified by preparative HPLC and their respective spectrum was confirmed by analytical HPLC. Active anti-cancer fraction was separately purified by preparative HPLC and the cytotoxicity result was shown with 100 µg/mL concentration. The anti-cancer compound of pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3 was detected by LC-MS analysis after interpretation with GC–MS data. Further, the morphological damages and increased proliferation with more death cells of pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 treated MCF-7 were observed by phase contrast and fluorescence microscope techniques. Finally, the nuclear condensation and increased necrotic cells of the pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 treated MCF-7 cells was clearly identified by fluorescence microscope techniques using Hoechst 33,342 stain. Therefore, our result was prove, the pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 rich Streptomyces akiyoshiensis GRG 6 (KY457710) as a potential anti-cancer agent against MCF-7 breast cancer cells.

【 授权许可】

Unknown