Genomic Evidence for Cryptic Speciation in Tree Frogs From the Apennine Peninsula, With Description of Hyla perrini sp. nov

Frontiers in Ecology and Evolution

Spartak N. Litvinchuk⁴  Daniele Canestrelli⁵  Amaël Borzée⁵  Tiziano Maddalena⁵  Alan Brelsford⁵  Paul A. Saunders⁵  Caroline Betto-Colliard⁵  Agnes Horn⁵  Romain Savary⁵  Guillaume Fabre⁵  Olivier Blaser⁵  Barret C. Phillips⁵  Karim Ghali⁵  Guillaume Lavanchy⁵  Christine Grossen⁵  Glib Mazepa⁵  Julien Leuenberger⁵  Nicolas Rodrigues⁶  Elisa Cavoto⁷  Daniel L. Jeffries⁸  Christophe Dufresnes⁸  Roberto Sermier⁹  Sylvain Dubey^1,10  Matthias Stöck^1,11 

[1] Associati sagl, Gordevio, Switzerland;WeberSA, Montreux, Switzerland;Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United Kingdom;Department of Biology, University of California, Riverside, Riverside, CA, United States;Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland;Department of Ecology and Genetics, Evolutionary Biology, Uppsala, Sweden;Division of EcoScience and Department of Life Sciences, Ewha Womans University, Seoul, South Korea;;Hintermann &Institute of Cytology, Russian Academy of Sciences, Saint Petersburg, Russia;Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Berlin, Germany;;Maddalena &

关键词: amphibians;    bioacoustics;    hybrid zone;    phylogenomics;    RAD-seq;    taxonomy;   

DOI  :  10.3389/fevo.2018.00144

来源: DOAJ

【 摘 要 】

Despite increasing appreciation of the speciation continuum, delimiting and describing new species is a major yet necessary challenge of modern phylogeography to help optimize conservation efforts. In amphibians, the lack of phenotypic differences between closely-related taxa, their complex, sometimes unresolved phylogenetic relationships, and their potential to hybridize all act to blur taxonomic boundaries. Here we implement a multi-disciplinary approach to evaluate the nature of two deeply-diverged mitochondrial lineages previously documented in Italian tree frogs (Hyla intermedia s. l.), distributed north and south of the Northern Apennine Mountains. Based on evidence from mitochondrial phylogenetics, nuclear phylogenomics, hybrid zone population genomics, niche modeling analyses, and biometric assessments, we propose that these lineages be considered distinct, cryptic species. Both mitochondrial and nuclear data affirm that they belong to two monophyletic clades of Pliocene divergence (~3.5 My), only admixing over a relatively narrow contact zone restricted to the southeast of the Po Plain (50–100 km). These characteristics are comparable to similarly-studied parapatric amphibians bearing a specific status. Inferred from their current geographic distribution, the two Italian tree frogs feature distinct ecological niches (<15% of niche overlap), raising questions regarding potential adaptive components contributing to their incipient speciation. However, we found no diagnostic morphological and bioacoustic differences between them. This system illustrates the speciation continuum of Western-Palearctic tree frogs and identifies additional cryptic lineages of similar divergence to be treated as separate species (H. cf. meridionalis). We recommend combined approaches using genomic data as applied here for the future taxonomic assessment of cryptic diversity in alloparapatric radiations of terrestrial vertebrates, especially in controversial taxa. Finally, we formally described the northern Italian tree frogs as a new species, Hyla perrini sp. nov.urn:lsid:zoobank.org:pub:C446334C-3D94-4820-A851-99BD95084F6D

【 授权许可】

Unknown