Nucleic acids through condensation of nucleosides and phosphorous acid in the presence of sulfur

Beilstein Journal of Organic Chemistry

Tuomas Lönnberg¹ 

[1] Department of Chemistry, University of Turku, Vatselankatu 2, FIN-20014 Turku, Finland;

关键词: H-phosphonate;    nucleic acid;    polymerization;    phosphite;    sulfurization;   

DOI  :  10.3762/bjoc.12.67

来源: DOAJ

【 摘 要 】

Short phosphorothioate oligonucleotides have been prepared by refluxing an equimolar mixture of thymidine and triethylammonium phosphite in toluene in the presence of elemental sulfur. Desulfurization and subsequent digestion of the products by P1 nuclease revealed that nearly 80% of the internucleosidic linkages thus formed were of the canonical 3´,5´-type.

【 授权许可】

Unknown