BIN1 protein isoforms are differentially expressed in astrocytes, neurons, and microglia: neuronal and astrocyte BIN1 are implicated in tau pathology

Molecular Neurodegeneration

Julie Schneider¹  David A. Bennett¹  Galina Marsh²  Andrea Crotti²  Charles White³  Yiyi Ma⁴  Alexandra Kroshilina⁴  Christina J. Yung⁴  Anthony Khairallah⁴  Elizabeth M. Bradshaw⁴  Hans-Ulrich Klein⁴  Marta Olah⁴  Mariko Taga⁴  Sarah M. Connor⁴  Philip L. De Jager⁴  Kyle Karhohs⁵  Anne E. Carpenter⁵  Vladislav A. Petyuk⁶  Richard Ransohoff⁷ 

[1] Alzheimer’s Disease Center, Rush University Medical Center;Biogen;Cell Circuits Program, Broad Institute;Center for Translational & Computational Neuroimmunology, Department of Neurology and the Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Columbia University Irving Medical Center;Imaging Platform, Broad Institute;Pacific Northwest National Laboratory;Third Rock Ventures;

关键词: BIN1 isoforms;    Alzheimer’s disease;    Amyloid;    Tau;    Microglia;    Astrocytes;   

DOI  :  10.1186/s13024-020-00387-3

来源: DOAJ

【 摘 要 】

Abstract Background Identified as an Alzheimer’s disease (AD) susceptibility gene by genome wide-association studies, BIN1 has 10 isoforms that are expressed in the Central Nervous System (CNS). The distribution of these isoforms in different cell types, as well as their role in AD pathology still remains unclear. Methods Utilizing antibodies targeting specific BIN1 epitopes in human post-mortem tissue and analyzing mRNA expression data from purified microglia, we identified three isoforms expressed in neurons and astrocytes (isoforms 1, 2 and 3) and four isoforms expressed in microglia (isoforms 6, 9, 10 and 12). The abundance of selected peptides, which correspond to groups of BIN1 protein isoforms, was measured in dorsolateral prefrontal cortex, and their relation to neuropathological features of AD was assessed. Results Peptides contained in exon 7 of BIN1’s N-BAR domain were found to be significantly associated with AD-related traits and, particularly, tau tangles. Decreased expression of BIN1 isoforms containing exon 7 is associated with greater accumulation of tangles and subsequent cognitive decline, with astrocytic rather than neuronal BIN1 being the more likely culprit. These effects are independent of the BIN1 AD risk variant. Conclusions Exploring the molecular mechanisms of specific BIN1 isoforms expressed by astrocytes may open new avenues for modulating the accumulation of Tau pathology in AD.

【 授权许可】

Unknown