Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3

Antioxidants

Zoran Vujčić¹  Biljana Dojnov²  Olga Radulović³  Marija Marković³  Milana Trifunović-Momčilov³  Slaviša Stanković⁴  Olja Stanojević⁴ 

[1] Department of Biochemistry, Faculty of Chemistry, University of Belgrade, 12-16 Studentski Trg, 11000 Belgrade, Serbia;Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade, 12 Njegoševa, 11000 Belgrade, Serbia;Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, 142 Bulevar Despota Stefana, 11060 Belgrade, Serbia;Faculty of Biology, University of Belgrade, 16 Studentski Trg, 11000 Belgrade, Serbia;

关键词: phenol;    bacteria;    duckweed;    antioxidative;    stress;   

DOI  :  10.3390/antiox10111719

来源: DOAJ

【 摘 要 】

Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L⁻¹), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei’s influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant’s defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.

【 授权许可】

Unknown