Omicron Booster in Ancestral Strain Vaccinated Mice Augments Protective Immunities Against Both Delta and Omicron Variants

Frontiers in Immunology

Wanhai Wang¹  Yifan Zhang¹  Dongmei Yan²  Shaoshuai Li²  Liqiu Jia³  Yang Zhou³  Yanmin Wan⁵  Chao Qiu⁶  Wenhong Zhang⁷ 

[1] Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Hehan, China;Department of Immunology, School of Basic Medical, Jiamusi University, Jiamusi, China;Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China;Department of Laboratory Medicine, Shanghai Public Health Clinical Center Fudan University, Shanghai, China;Department of Radiology, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China;;Institutes of Biomedical Sciences &Key Laboratory of Medical Molecular Virology (MOE/MOH), Shanghai Medical College, Fudan University, Shanghai, China;National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, China;

关键词: Omicron-matched vaccine;    booster vaccination;    cross-reactivity;    T cell;    antibody;   

DOI  :  10.3389/fimmu.2022.897879

来源: DOAJ

【 摘 要 】

A booster vaccination is called for constraining the evolving epidemic of SARS-CoV-2. However, the necessity of a new COVID-19 vaccine is currently unclear. To compare the effect of an Omicron-matched S DNA vaccine and an ancestral S DNA vaccine in boosting cross-reactive immunities, we firstly immunized mice with two-dose of a DNA vaccine encoding the spike protein of the ancestral Wuhan strain. Then the mice were boosted with DNA vaccines encoding spike proteins of either the Wuhan strain or the Omicron variant. Specific antibody and T cell responses were measured at 4 weeks post boost. Our data showed that the Omicron-matched vaccine efficiently boosted RBD binding antibody and neutralizing antibody responses against both the Delta and the Omicron variants. Of note, antibody responses against the Omicron variant elicited by the Omicron-matched vaccine were much stronger than those induced by the ancestral S DNA vaccine. Meanwhile, CD8+ T cell responses against both the ancestral Wuhan strain and the Omicron strain also tended to be higher in mice boosted by the Omicron-matched vaccine than those in mice boosted with the ancestral S DNA vaccine, albeit no significant difference was observed. Our findings suggest that an Omicron-matched vaccine is preferred for boosting cross-protective immunities.

【 授权许可】

Unknown