CD107a+ (LAMP-1) Cytotoxic CD8+ T-Cells in Lupus Nephritis Patients

Frontiers in Medicine

Oliver Witzke¹  Sebastian Dolff¹  Bartosz Tyczynski²  Andreas Kribben³  Benjamin Wilde³  Anika Wiechmann³  Kerstin Amann⁴  Wayel H. Abdulahad⁵  Karl Sebastian Lang⁷ 

[1] Department of Infectious Diseases, West German Centre of Infectious Diseases, University Duisburg-Essen, Essen, Germany;Department of Medical Intensive Care I, University Hospital Essen, University Duisburg-Essen, Essen, Germany;Department of Nephrology, University Duisburg-Essen, Essen, Germany;Department of Nephropathology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany;Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, Netherlands;Department of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, Netherlands;Institute of Immunology, Medical Faculty, University of Duisburg-Essen, Essen, Germany;

关键词: cytotoxic T-cells;    CD107a;    LAMP-1;    lupus nephritis;    SLE;   

DOI  :  10.3389/fmed.2021.556776

来源: DOAJ

【 摘 要 】

Cytotoxic CD8+ T-cells play a pivotal role in the pathogenesis of systemic lupus erythematosus (SLE). The aim of this study was to investigate the role of CD107a (LAMP-1) on cytotoxic CD8+ T-cells in SLE-patients in particular with lupus nephritis. Peripheral blood of SLE-patients (n = 31) and healthy controls (n = 21) was analyzed for the expression of CD314 and CD107a by flow cytometry. Kidney biopsies of lupus nephritis patients were investigated for the presence of CD8+ and C107a+ cells by immunohistochemistry and immunofluorescence staining. The percentages of CD107a+ on CD8+ T-cells were significantly decreased in SLE-patients as compared to healthy controls (40.2 ± 18.5% vs. 47.9 ± 15.0%, p = 0.02). This was even more significant in SLE-patients with inactive disease. There was a significant correlation between the percentages of CD107a+CD8+ T-cells and SLEDAI. The evaluation of lupus nephritis biopsies showed a significant number of CD107a+CD8+ T-cells mainly located in the peritubular infiltrates. The intrarenal expression of CD107a+ was significantly correlated with proteinuria. These results demonstrate that CD8+ T-cells of patients with systemic lupus erythematosus have an altered expression of CD107a which seems to be associated with disease activity. The proof of intrarenal CD107a+CD8+ suggests a role in the pathogenesis of lupus nephritis.

【 授权许可】

Unknown