Acquisition of pluripotency in the chick embryo occurs during intrauterine embryonic development via a unique transcriptional network

Journal of Animal Science and Biotechnology

Jae Yong Han¹  Hyo Gun Lee¹  Young Sun Hwang¹  Young Hyun Park¹  Sang Kyung Kim¹  Jeong Mook Lim¹  Deivendran Rengaraj²  Byung Wook Cho³ 

[1] Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University;Department of Animal Science and Technology, Chung-Ang University;Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University;

关键词: Avian;    Embryonic development;    NANOG;    Pluripotency;    Transcriptional factor;   

DOI  :  10.1186/s40104-018-0246-0

来源: DOAJ

【 摘 要 】

Abstract Background Acquisition of pluripotency by transcriptional regulatory factors is an initial developmental event that is required for regulation of cell fate and lineage specification during early embryonic development. The evolutionarily conserved core transcriptional factors regulating the pluripotency network in fishes, amphibians, and mammals have been elucidated. There are also species-specific maternally inherited transcriptional factors and their intricate transcriptional networks important in the acquisition of pluripotency. In avian species, however, the core transcriptional network that governs the acquisition of pluripotency during early embryonic development is not well understood. Results We found that chicken NANOG (cNANOG) was expressed in the stages between the pre-ovulatory follicle and oocyte and was continuously detected in Eyal-Giladi and Kochav stage I (EGK.I) to X. However, cPOUV was not expressed during folliculogenesis, but began to be detectable between EGK.V and VI. Unexpectedly, cSOX2 could not be detected during folliculogenesis and intrauterine embryonic development. Instead of cSOX2, cSOX3 was maternally inherited and continuously expressed during chicken intrauterine development. In addition, we found that the pluripotency-related genes such as cENS-1, cKIT, cLIN28A, cMYC, cPRDM14, and cSALL4 began to be dramatically upregulated between EGK.VI and VIII. Conclusion These results suggest that chickens have a unique pluripotent circuitry since maternally inherited cNANOG and cSOX3 may play an important role in the initial acquisition of pluripotency. Moreover, the acquisition of pluripotency in chicken embryos occurs at around EGK.VI to VIII.

【 授权许可】

Unknown