3D Cultures of Salivary Gland Cells in Native or Gelled Egg Yolk Plasma, Combined with Egg White and 3D-Printing of Gelled Egg Yolk Plasma

Materials

AndréM. Charbonneau¹  SimonD. Tran¹  JosephM. Kinsella² 

[1] Faculty of Dentistry, Craniofacial Tissue Engineering and Stem Cells Laboratory, McGill University, Montréal, QC H3A 0C7, Canada;Faculty of Engineering, Department of Bioengineering, McGill University, Montréal, QC H3A 0C7, Canada;

关键词: 3d-bioprinting;    3d-printing;    salivary glands;    3d-cryo well insert;    histology;    egg yolk plasma;    egg white;    gel;    ki-67;    rheology;    cell culture;    tissue engineering;   

DOI  :  10.3390/ma12213480

来源: DOAJ

【 摘 要 】

For salivary gland (SG) tissue engineering, we cultured acinar NS-SV-AC cell line or primary SG fibroblasts for 14 days in avian egg yolk plasma (EYP). Media or egg white (EW) supplemented the cultures as they grew in 3D-Cryo histology well inserts. In the second half of this manuscript, we measured EYP’s freeze-thaw gelation and freeze-thaw induced gelled EYP (_GEYP), and designed and tested further _GEYP tissue engineering applications. With a 3D-Cryo well insert, we tested _GEYP as a structural support for 3D cell culture or as a bio-ink for 3D-Bioprinting fluorescent cells. In non-printed EYP + EW or _GEYP + EW cultures, sagittal sections of the cultures showed cells remaining above the well’s base. Ki-67 expression was lacking for fibroblasts, contrasting NS-SV-AC’s constant expression. Rheological viscoelastic measurements of _GEYP at 37 °C on seven different freezing periods showed constant increase from 0 in mean storage and loss moduli, to 320 Pa and 120 Pa, respectively, after 30 days. We successfully 3D-printed _GEYP with controlled geometries. We manually extruded _GEYP bio-ink with fluorescence cells into a 3D-Cryo well insert and showed cell positioning. The 3D-Cryo well inserts reveal information on cells in EYP and we demonstrated _GEYP cell culture and 3D-printing applications.

【 授权许可】

Unknown