Junctional Modulation of Round Window Membrane Enhances Dexamethasone Uptake into the Inner Ear and Recovery after NIHL

International Journal of Molecular Sciences

Tae Hwan Kim¹  Akanksha Gajibhiye²  Ah-Ra Lyu²  Seong-Hun Jeong²  Yong-Ho Park²  Yoonjoong Kim³  Yang Yu⁴  Min Jung Park⁴  Sun-Ae Shin⁴  Yongde Jin⁵  A Reum Je⁶  Yang Hoon Huh⁶ 

[1] Biomedical Research Institute, Chungnam National University Hospital, Daejeon 35015, Korea;Department of Medical Science, Chungnam National University, Daejeon 35015, Korea;Department of Otolaryngology—Head and Neck Surgery, Chungbuk National University Hospital, Cheongju 28644, Korea;Department of Otolaryngology—Head and Neck Surgery, Chungnam National University, Daejeon 35015, Korea;Department of Otolaryngology—Head and Neck Surgery, Yanbian University Hospital, Yanji 133000, China;Electron Microscopy Research Center, Korea Basic Science Institute, Cheongju 28116, Korea;

关键词: cochlea;    round window membrane;    RWM;    noise-induced hearing loss;    NIHL;    sodium caprate;   

DOI  :  10.3390/ijms221810061

来源: DOAJ

【 摘 要 】

Delivery of substances into the inner ear via local routes is increasingly being used in clinical treatment. Studies have focused on methods to increase permeability through the round window membrane (RWM) and enhance drug diffusion into the inner ear. However, the clinical applications of those methods have been unclear and few studies have investigated the efficacy of methods in an inner ear injury model. Here, we employed the medium chain fatty acid caprate, a biologically safe, clinically applicable substance, to modulate tight junctions of the RWM. Intratympanic treatment of sodium caprate (SC) induced transient, but wider, gaps in intercellular spaces of the RWM epithelial layer and enhanced the perilymph and cochlear concentrations/uptake of dexamethasone. Importantly, dexamethasone co–administered with SC led to significantly more rapid recovery from noise-induced hearing loss at 4 and 8 kHz, compared with the dexamethasone-only group. Taken together, our data indicate that junctional modulation of the RWM by SC enhances dexamethasone uptake into the inner ear, thereby hastening the recovery of hearing sensitivity after noise trauma.

【 授权许可】

Unknown