Molecular Diversity and Compositional Analysis of Microbiota in Aged Refuse Biofilter Revealed by Amplicon Sequencing

Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ

HasfalinaChe Man¹  Nik NorsyahariatiNik Daud²  Sandra C.Anijiofor³ 

[1] Department of Biological and Agricultural Engineering, Faculty of Engineering, Universiti Putra Malaysia, Selangor 43400, Malaysia;Department of Civil Engineering, Faculty of Engineering, Universiti Putra Malaysia, Selangor 43400, Malaysia;Department of Civil Engineering, Federal Polytechnic Birnin Kebbi, PMB 1034, Nigeria;

关键词: Diversity;    microbial community;    aged refuse biofilter;    Commamox;    nitrification;    metagenomics;   

DOI  :  10.37077/25200860.2021.34.sp1.14

来源: DOAJ

【 摘 要 】

The increasing significance of aged refuse biofilters for biodegradation processes in wastewater treatment necessitated the constant search for its bacterial properties. This study focused on the diversity of the bacteria present in aged refuse by DNA extraction. The PCR amplifier was used for DNA sequencing using DNA polymerase primer. For PCR amplicons purification, amplicons were extracted from 2% agarose gels and purified using DNA Gel Extraction Kit while quantification was done using QuantiFluor™ -ST. Sample libraries were pooled in equimolar and paired-end sequenced on an Illumina MiSeq platform while Raw fastq files were demultiplexed, quality-filtered using QIIME. The taxonomy of each 16S rRNA gene sequence was evaluated by RDP using confidence threshold of 0.7 while operational Units (OTUs) were clustered with 97% similarity cut-off using UPARSE. The result revealed twelve phyla clusters which include; Firmicutes, Chloroflexi, Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, Planctomycetes, Nitrospirae, Cyanobacteria, Latescibacteria and Gemmatimonadetes. Furthermore, additional group classification revealed up to 513 reads of bacteria kingdom with dominant family classification of Anaerolineaceae, Ruminococcaceae, Nitrosomonadaceae, Planctomycetaceae, Clostridiaceae, Beijerinkiaceae, Xanthobacteracaea, Veillonellacaea, Cemmatimonadacaea. The presence of such diverse organisms in the aged refuse biofilter, show that the aged refuse biofilter has biodegradation potentials when treatment conditions are efficiently optimized.

【 授权许可】

Unknown