Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review

BioResources

Lin Chen¹  Jun Wang²  Liang Zhu³  Mei Pan⁴  Yunpeng Qiu⁴ 

[1] China;College of Environment, Hohai University, Nanjing 210098, China;College of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224003, China;State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China;

关键词: Extracellular polymeric substance;    Biofilm;    Detection technique;    Multidisciplinary methods;   

DOI  :  10.15376/biores.11.3.8092-8115

来源: DOAJ

【 摘 要 】

Extracellular polymeric substances (EPS) are one of the main components of biofilm, prompting biofilm to form a cohesive three-dimensional framework. Numerous methods are available to help characterize the properties and the structural, chemical and physical organizations of EPS during the biofilm formation process. This review highlights key techniques from different disciplines that have been successfully applied in-situ and non-destructively to describe the complex composition and distribution of EPS in biofilm, especially microscopic, spectroscopic, and the combination of multi-disciplinary methods that can provide new insights into the complex structure/function correlations in biofilms. Among them, confocal laser scanning microscopy (CLSM) is emphasized, and its principles, applications, advantages, and limitations are summarized. Multidisciplinary techniques have been developed and recommended to study EPS during the biofilm formation process, providing more in-depth insights into the composition and spatial distributions of EPS, so as to improve our understanding of the role EPS plays in biofilms ultimately.

【 授权许可】

Unknown