Influence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs

Animals

Anders Johannisson¹  Jane M. Morrell¹  Osvaldo Bogado Pascottini²  Guillaume Domain²  Ann Van Soom²  Hiba Ali Hassan²  Eline Wydooghe²  Wojciech Niżański³ 

[1] Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, 756-51 Uppsala, Sweden;Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium;Department of Reproduction and Clinic of Farm Animals, University of Environmental Science, Grundwaldzki Square 49, 50-357 Wroclaw, Poland;

关键词: canine;    fertility;    semen selection;    cryopreservation;    semen quality;   

DOI  :  10.3390/ani12060714

来源: DOAJ

【 摘 要 】

This study evaluated how semen selection by single layer centrifugation (SLC) with Canicoll affects semen freezability in dogs. A total of eighteen ejaculates, collected from dogs with optimal and suboptimal semen quality (optimal: normal morphology (NM) ≥ 80%, n = 9; suboptimal: NM between 60 and 79%, n = 9), were divided into two aliquots and subjected to standard centrifugation or SLC before cryopreservation. Motility, NM, membrane integrity, mitochondrial membrane potential (MMP), and DNA integrity were improved in fresh samples after SLC, regardless of semen quality, but at the expense of some good quality spermatozoa. After thawing, NM and membrane integrity were improved in SLC-selected semen in both semen qualities. Interestingly, MMP was also higher but only in optimal quality semen. Still, spermatozoa from suboptimal quality semen did not survive freezing to the same extent as spermatozoa from optimal quality semen, even after selecting superior spermatozoa. Semen selection with Canicoll is, therefore, an effective technique to isolate a subpopulation of high-quality spermatozoa and obtain sperm samples of better quality after thawing, but is not sufficient to improve the intrinsic inferior freezability of suboptimal quality semen. So far, eighteen pups were born after insemination with SLC-selected frozen-thawed semen, proving that these selected spermatozoa remain fertile.

【 授权许可】

Unknown