Genome-Wide Mapping of Growth-Related Quantitative Trait Loci in Orange-Spotted Grouper (Epinephelus coioides) Using Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq)

International Journal of Molecular Sciences

Jia Li¹  Hui Yu¹  Qiong Shi¹  Haifa Zhang²  Hankui Liu³  Xinxin You³  Ling Xiao⁴  Hao-Ran Lin⁴  Zining Meng⁴  Yong Zhang⁴ 

[1] BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China;Marine Fisheries Development Center of Guangdong Province, Huizhou 510610, China;Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, State Key Laboratory of Agricultural Genomics, BGI, Shenzhen 518083, China;State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China;

关键词: quantitative trait loci (QTL);    genetic linkage map;    growth-related genes;    ddRADseq;    orange-spotted grouper (Epinephelus coioides);   

DOI  :  10.3390/ijms17040501

来源: DOAJ

【 摘 要 】

Mapping of quantitative trait loci (QTL) is essential for the discovery of genetic structures that related to complex quantitative traits. In this study, we identified 264,072 raw SNPs (single-nucleotide polymorphisms) by double digest restriction site associated DNA sequencing (ddRADseq), and utilized 3029 of these SNPs to construct a genetic linkage map in orange-spotted grouper (Epinephelus coioides) using a regression mapping algorithm. The genetic map contained 24 linkage groups (LGs) spanning a total genetic distance of 1231.98 cM. Twenty-seven significant growth-related QTLs were identified. Furthermore, we identified 17 genes (fez2, alg3, ece2, arvcf, sla27a4, sgk223, camk2, prrc2b, mchr1, sardh, pappa, syk, tert, wdrcp91, ftz-f1, mate1 and notch1) including three (tert, ftz-f1 and notch1) that have been reported to be involved in fish growth. To summarize, we mapped growth-related QTLs in the orange-spotted grouper. These QTLs will be useful in marker-assisted selection (MAS) efforts to improve growth-related traits in this economically important fish.

【 授权许可】

Unknown