The Differential Effect of Cannabidiol on the Composition and Physicochemical Properties of Keratinocyte and Fibroblast Membranes from Psoriatic Patients and Healthy People

Membranes

Wojciech Łuczaj¹  Anna Jastrząb¹  Adam Wroński²  Izabela Dobrzyńska³  Barbara Szachowicz-Petelska³ 

[1] Department of Analytical Chemistry, Medical University of Białystok, Mickiewicza 2d, 15-222 Białystok, Poland;Dermatological Specialized Center ”DERMAL” NZOZ in Białystok, Nowy Swiat 17/5, 15-453 Białystok, Poland;Faculty of Chemistry, University in Białystok, Ciołkowskiego 1K, 15-245 Białystok, Poland;

关键词: psoriasis;    cannabidiol;    UV radiation;    fibroblasts;    keratinocytes;    electrical properties;   

DOI  :  10.3390/membranes11020111

来源: DOAJ

【 摘 要 】

The development of psoriasis is accompanied by oxidative stress, which can modify the components of skin cells. Therefore, the aim of this study was to evaluate the effect of cannabidiol (CBD), an antioxidant and anti-inflammatory phytocannabinoid, on the composition and physicochemical properties of the membranes of healthy and psoriatic keratinocytes and fibroblasts exposed to ultraviolet A (UVA) and ultraviolet B (UVB) radiation. In psoriasis-altered cells, decreased levels of the main groups of phospholipids and increased levels of sialic acid and malondialdehyde (MDA), a lipid peroxidation product, as well as negative charge of cell membranes compared to non-diseased cells, were found. On the other hand, UVA/B radiation increased the levels of phospholipids and MDA in both groups of cells. Moreover, psoriatic cells were characterized by lower levels of sialic acid and negative charge of cell membranes, while non-diseased cells showed the opposite response. The CBD treatment intensified some of the changes (phospholipid content and membrane charge) caused by the radiation of psoriatic cells, while it prevented these changes in the cells of healthy people. The results of this study indicate that CBD can prevent structural and functional changes to the membranes of healthy skin cells during phototherapy for psoriasis.

【 授权许可】

Unknown