Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

Applied Sciences

Marina Nisnevitch¹  Abdelhamid Errachid²  GalinaZ. Gayda³  OlhaM. Demkiv³  RomanYa. Serkiz³  NataliyaYe. Stasyuk³  MykhailoV. Gonchar³  MaksymD. Lootsik³ 

[1] Department of Chemical Engineering, Biotechnology and Materials, Ariel University, Ariel 4070000, Israel;Institute of Analytical Sciences, UMR 5280, CNRS, Université Claude Bernard Lyon 1, ENS Lyon-5, 5 rue de la Doua, F-69100 Villeurbanne, France;Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street 14/16, 79005 Lviv, Ukraine;

关键词: green synthesis;    metallic nanoparticles;    extracellular metabolites;    yeast Ogataea polymorpha;    laccase;    alcohol oxidase;    amperometric biosensors;   

DOI  :  10.3390/app9040720

来源: DOAJ

【 摘 要 】

Novel nanomaterials, including metallic nanoparticles obtained via green synthesis (gNPs), have a great potential for application in biotechnology, industry and medicine. The special role of gNPs is related to antibacterial agents, fluorescent markers and carriers for drug delivery. However, application of gNPs for construction of amperometric biosensors (ABSs) is not well documented. The aim of the current research was to study potential advantages of using gNPs in biosensorics. The extracellular metabolites of the yeast Ogataea polymorpha were used as reducing agents for obtaining gNPs from the corresponding inorganic ions. Several gNPs were synthesized, characterized and tested as enzyme carriers on the surface of graphite electrodes (GEs). The most effective were Pd-based gNPs (gPdNPs), and these were studied further and applied for construction of laccase- and alcohol oxidase (AO)-based ABSs. AO/GE, AO-gPdNPs/GE, laccase/GE and laccase-gPdNPs/GE were obtained, and their analytical characteristics were studied. Both gPdNPs-modified ABSs were found to have broader linear ranges and higher storage stabilities than control electrodes, although they are less sensitive toward corresponding substrates. We thus conclude that gPdNPs may be promising for construction of ABSs for enzymes with very high affinities to their substrates.

【 授权许可】

Unknown